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pVZ_PcoaT_SQE1_MRN1
(Plasmid #103858)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 103858 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVZ-spec
  • Backbone manufacturer
    Sergey V. Shestakov and Anne Karradt and Wolfgang Lockau
  • Modifications to backbone
    pVZ-spec is a modified version of pVZ321 (Sergey V. Shestakov) made by Anne Karradt in the lab of Wolfgang Lockau.
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin and Spectinomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    marneral synthase 1
  • Alt name
    MRN1
  • Alt name
    At5g42600
  • Species
    A. thaliana (mustard weed)
  • Mutation
    inserted genes are codon optimized2nd amino acid of SQE1 changed from Glu to Ala
  • Entrez Gene
    MRN1 (a.k.a. AT5G42600, MFO20.1, MFO20_1, marneral synthase, marneral synthase 1)
  • Promoter PcoaT (from Synechocystis sp. PCC 6803)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

second gene: squalene epoxidase (SQE1)
third gene: coaR repressor protein from Synechoccystis sp. PCC6803
growth temperature (37C) referes to E. coli ; in Synechoccystis sp. PCC6803: 30 C

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVZ_PcoaT_SQE1_MRN1 was a gift from Ilka Axmann (Addgene plasmid # 103858 ; http://n2t.net/addgene:103858 ; RRID:Addgene_103858)

  • For your References section:

    The photosynthetic bacteria Rhodobacter capsulatus and Synechocystis sp. PCC 6803 as new hosts for cyclic plant triterpene biosynthesis. Loeschcke A, Dienst D, Wewer V, Hage-Hulsmann J, Dietsch M, Kranz-Finger S, Huren V, Metzger S, Urlacher VB, Gigolashvili T, Kopriva S, Axmann IM, Drepper T, Jaeger KE. PLoS One. 2017 Dec 27;12(12):e0189816. doi: 10.1371/journal.pone.0189816. eCollection 2017. 10.1371/journal.pone.0189816 PubMed 29281679
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