pVZ_PcoaT_SQE1_CAS1
(Plasmid #103856)

• Purpose: Heterologous, cobalt-inducible expression of SQE1 and CAS1 from A. thaliana in Synechocystis sp. PCC6803
• Depositing Lab: Ilka Axmann
• Publication: Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. doi: 10.1371/journal.pone.0189816. eCollection 2017.

Backbone

• Vector backbone: pVZ-spec
• Backbone manufacturer: Sergey V. Shestakov and Anne Karradt and Wolfgang Lockau
• Modifications to backbone: pVZ-spec is a modified version of pVZ321 (Sergey V. Shestakov) made by Anne Karradt in the lab of Wolfgang Lockau.
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin and Spectinomycin, 50 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: cycloartenol synthase 1
• Alt name: CAS1
• Alt name: At2g07050
• Species: A. thaliana (mustard weed)
• Mutation: inserted genes are codon optimized. 2nd amino acid of SQE1 changed from Glu to Ala
• Entrez Gene: CAS1 (a.k.a. AT2G07050, CYCLOARTENOL SYNTHASE 1, T4E14.16, T4E14_16, cycloartenol synthase 1)
• Promoter: PcoaT (from Synechocystis sp. PCC 6803)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pVZ_PcoaT_SQE1_CAS1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

second gene: squalene epoxidase (SQE1)
third gene: coaR repressor protein from Synechoccystis sp. PCC6803
growth temperature (37C) referes to E. coli ; in Synechoccystis sp. PCC6803: 30 C