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Addgene

pC0044-EF1a-RanCas13b-NES-mapk
(Plasmid #103855)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 103855 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EF1a-lenti
  • Backbone size w/o insert (bp) 9000
  • Total vector size (bp) 12621
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RanCas13b
  • Species
    Riemerella anatipestifer
  • Insert Size (bp)
    3285
  • GenBank ID
    WP_004919755
  • Tags / Fusion Proteins
    • mapk NES (C terminal on insert)
    • 3xHA (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC0044-EF1a-RanCas13b-NES-mapk was a gift from Feng Zhang (Addgene plasmid # 103855 ; http://n2t.net/addgene:103855 ; RRID:Addgene_103855)

  • For your References section:

    RNA editing with CRISPR-Cas13. Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180. 10.1126/science.aaq0180 PubMed 29070703
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