pC0043-PspCas13b crRNA backbone
(Plasmid #103854)

• Purpose: For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac
• Depositing Lab: Feng Zhang
• Publication: Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2686
• Total vector size (bp): 2962
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: U6-PspCas13b DR-BbsI-BbsI-polyT
• Insert Size (bp): 314

Resource Information

• Supplemental Documents:
  • pc0043-pspcas13b-crrna-backbone.gb
• Articles Citing this Plasmid:
  • 28 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA - COVID 19 Research

Depositor Comments

Benchling link: https://benchling.com/s/seq-OH6nMmZCZn930BWqcFNa

Information for Cloning Grade DNA (Catalog # 103854-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA - COVID 19 Research

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here