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Addgene

pHES553 [prGal1-RAS2(KAN) pnh604]
(Plasmid #103846)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 103846 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNH604
  • Total vector size (bp) 6500
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pGal1-Ras2(KAN)
  • Species
    S. cerevisiae (budding yeast)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHES553 [prGal1-RAS2(KAN) pnh604] was a gift from Hana El-Samad (Addgene plasmid # 103846 ; http://n2t.net/addgene:103846 ; RRID:Addgene_103846)

  • For your References section:

    Model-guided optogenetic study of PKA signaling in budding yeast. Stewart-Ornstein J, Chen S, Bhatnagar R, Weissman JS, El-Samad H. Mol Biol Cell. 2017 Jan 1;28(1):221-227. doi: 10.1091/mbc.E16-06-0354. Epub 2016 Nov 9. 10.1091/mbc.E16-06-0354 PubMed 28035051
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