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ptagBFP-LacI
(Plasmid #103839)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 103839 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tagBFP-LacI
  • Insert Size (bp)
    1815
  • Mutation
    LacI: C19T (silent, L7L), T264C (silent, A88A), C370A (L124I), C404T (T135I), A417T (silent, A139A), G424A (A142T), T465A (silent, T155T)
  • Promoter SV2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

replaced CMV promoter by SV2 promoter and EGFP by tagBFP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ptagBFP-LacI was a gift from Karsten Rippe (Addgene plasmid # 103839 ; http://n2t.net/addgene:103839 ; RRID:Addgene_103839)

  • For your References section:

    Real-time observation of light-controlled transcription in living cells. Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K. J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. Epub 2017 Nov 9. 10.1242/jcs.205534 PubMed 29122982
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