pmCherry-MS2coatProtein
(Plasmid #103832)

• Purpose: Live cell reader for MS2 loop RNA production
• Depositing Lab: Karsten Rippe
• Publication: Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. Epub 2017 Nov 9.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3941
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry-MS2 coat protein
• Insert Size (bp): 1102
• Mutation: MCP: nt 202-240 are missing (13 aa), C242G (A81G), A246C (silent, A82A), T291C (silent, A97A)
• Entrez Gene: cp (a.k.a. MS2g2)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • Annotated sequence file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

EGFP was cut out from the backbone (AgeI/BamHI cuts); relevance of mutations unknown, but insert sequence is almost identical (except A88G, I30V) to another published MCP sequence (GenBank: JQ624676.1, Auslaender et al. 2012, Nature 487(7405): 123-127)