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Addgene

pPHR-EYFP-hGCN5mut
(Plasmid #103828)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 103828 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3935
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PHR-EYFP-hGCN5mut
  • Alt name
    PHR domain of CRY2
  • Species
    H. sapiens (human), A. thaliana (mustard weed)
  • Insert Size (bp)
    4755
  • Mutation
    hGCN5: A566G (E189G), G585T (K195N), G726A (silent, K242K), 1861-1866: TACTTC -> GCAGCA (Y621A, F622A)
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
  • Entrez Gene
    KAT2A (a.k.a. GCN5, GCN5L2, PCAF-b, hGCN5)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mCherry was cut out from the backbone (XhoI/XbaI cuts); Y621A and F622A mutations ablate KAT activity (Lerin et al. 2006), amplified by PCR from Addgene #14425

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPHR-EYFP-hGCN5mut was a gift from Karsten Rippe (Addgene plasmid # 103828 ; http://n2t.net/addgene:103828 ; RRID:Addgene_103828)

  • For your References section:

    Real-time observation of light-controlled transcription in living cells. Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K. J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. Epub 2017 Nov 9. 10.1242/jcs.205534 PubMed 29122982
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