pPHR-EYFP-NLS-VP16
(Plasmid #103822)

• Purpose: Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination
• Depositing Lab: Karsten Rippe
• Publication: Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. Epub 2017 Nov 9.

Backbone

• Vector backbone: pmCherry-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3935
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PHR-EYFP-VP16
• Alt name: PHR domain of CRY2 & transactivation domain of UL48 (VP16)
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 2566
• Mutation: VP16: G126T (silent, G42G)
• Entrez Gene: CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XbaI (destroyed during cloning)
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • Annotated sequence file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

aa412-482 of UL48 (transactivation domain); mCherry was cut out from the backbone (XhoI/XbaI cuts)