pCIBN-TetR
(Plasmid #103813)

• Purpose: BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light
• Depositing Lab: Karsten Rippe
• Publication: Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. Epub 2017 Nov 9.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3932
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CIBN-TetR
• Species: A. thaliana (mustard weed); E. coli
• Insert Size (bp): 1214
• Mutation: TetR: A4G (M2V)
• Entrez Gene: CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
• Entrez Gene: tetR (a.k.a. TC1_0054)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • Annotated sequence file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

EGFP was cut out from the backbone (AgeI/XbaI cuts); relevance of mutations unknown