pCIBN-LacI-tagRFP-T
(Plasmid #103810)

• Purpose: 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
• Depositing Lab: Karsten Rippe
• Publication: Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. Epub 2017 Nov 9.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3932
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CIBN-LacI-tagRFP-T
• Alt name: N-terminus of CIB1
• Species: A. thaliana (mustard weed); E. coli
• Insert Size (bp): 2337
• Mutation: LacI: C19T (silent, L7L), T264C (silent, A88A), C370A (L124I), C404T (T135I), A417T (silent, A139A), G424A (A142T), T465A (silent, T155T)
• Entrez Gene: CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
• Entrez Gene: lacI (a.k.a. O3K_19750)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • Annotated sequence file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

EGFP was cut out from the backbone (NheI/BamHI cuts); relevance of mutations unknown