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Addgene

pCRA03
(Plasmid #103141)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 103141 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    dCas9v2
  • Total vector size (bp) 13951
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    gRNAs
  • gRNA/shRNA sequence
    gttcactgccgtataggcagctaagaaaCGCCCCAGTTGGCTTGGCTCgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtggtgcGTTCACTGCCGTATAGGCAGCTAAGAAAAGCTGTTGTTCGATAAATCCgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtggtgcgttcactgccgtataggcagctaagaaaTAATGGCATCGGAGGCTCGGgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtggtgcgttcactgccgtataggcagctaagaaa
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer agagatggcgcacatggt
  • 3′ sequencing primer atccaaacaaaattcaatagttac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRA03 was a gift from Jens Nielsen (Addgene plasmid # 103141 ; http://n2t.net/addgene:103141 ; RRID:Addgene_103141)

  • For your References section:

    Multiplexed CRISPR/Cas9 Genome Editing and Gene Regulation using Csy4 in Saccharomyces cerevisiae. Ferreira R, Skrekas C, Nielsen J, David F. ACS Synth Biol. 2017 Nov 21. doi: 10.1021/acssynbio.7b00259. 10.1021/acssynbio.7b00259 PubMed 29161506
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