Skip to main content
Addgene

p413_Csy4NLS
(Plasmid #103139)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 103139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    p413 TEF
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 5522
  • Total vector size (bp) 6130
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Csy4
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    564
  • GenBank ID
    4AL5
  • Promoter TEF
  • Tag / Fusion Protein
    • NLS (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGGTCTTCAATTTCTCAAGT
  • 3′ sequencing primer CTCCTTCCTTTTCGGTTA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p413_Csy4NLS was a gift from Jens Nielsen (Addgene plasmid # 103139 ; http://n2t.net/addgene:103139 ; RRID:Addgene_103139)

  • For your References section:

    Multiplexed CRISPR/Cas9 Genome Editing and Gene Regulation using Csy4 in Saccharomyces cerevisiae. Ferreira R, Skrekas C, Nielsen J, David F. ACS Synth Biol. 2017 Nov 21. doi: 10.1021/acssynbio.7b00259. 10.1021/acssynbio.7b00259 PubMed 29161506
Related items:
Commonly requested with: