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Addgene

pcDNA-f:PGC1b
(Plasmid #1031)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1031 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA-flag
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGC-1 beta
  • Alt name
    PGC1beta
  • Alt name
    PGC-1b
  • Alt name
    PPAR gamma coactivator 1 beta
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3660
  • GenBank ID
    NM_133249
  • Entrez Gene
    Ppargc1b (a.k.a. 4631412G21Rik, PGC-1beta, PGC-1beta/ERRL1, PPARGC-1-beta, Perc)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-f:PGC1b was a gift from Bruce Spiegelman (Addgene plasmid # 1031 ; http://n2t.net/addgene:1031 ; RRID:Addgene_1031)

  • For your References section:

    Peroxisome proliferator-activated receptor gamma coactivator 1beta (PGC-1beta ), a novel PGC-1-related transcription coactivator associated with host cell factor. Lin J, Puigserver P, Donovan J, Tarr P, Spiegelman BM. J Biol Chem 2002 Jan 18;277(3):1645-8. Epub 2001 Nov 30. 10.1074/jbc.C100631200 PubMed 11733490
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