Skip to main content
Addgene

pKSJ375
(Plasmid #103064)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 103064 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET52-b
  • Backbone manufacturer
    Novagen/EMD Millipore
  • Backbone size w/o insert (bp) 5227
  • Total vector size (bp) 5700
  • Vector type
    Bacterial Expression
  • Selectable markers
    Immunity to colicin E1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21(DE3)
  • Growth instructions
    use BL21(DE3) for protein expression
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    colicin E1 immunity protein
  • Alt name
    cei
  • Species
    E. coli
  • Insert Size (bp)
    440
  • GenBank ID
    J01566.1
  • Entrez Gene
    pColE1_26 (a.k.a. pColE1_26)
  • Entrez Gene
    imm (a.k.a. pColE1H22_p2)
  • Promoter E1 immunity protein promoter from ColE1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvrII (not destroyed)
  • 3′ cloning site AvrII (not destroyed)
  • 5′ sequencing primer T7-term
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This protein was never expressed independently. It was cloned in order to subsequently clone a colicin gene, which would be lethal in the absence of the immunity protein. The cloned fragment was from base pair 500 to base pair 48 of ColE1, which encompasses the E1 immunity protein promoter and the gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKSJ375 was a gift from Alan Finkelstein & Karen Jakes (Addgene plasmid # 103064 ; http://n2t.net/addgene:103064 ; RRID:Addgene_103064)

  • For your References section:

    The Colicin E1 TolC Box: Identification of a Domain Required for Colicin E1 Cytotoxicity and TolC Binding. Jakes KS. J Bacteriol. 2016 Dec 13;199(1). pii: e00412-16. Print 2017 Jan 1. 10.1128/JB.00412-16 PubMed 27795317
Related items: