pSC101_TIMER
(Plasmid #103057)

• Purpose: low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)
• Depositing Lab: Dirk Bumann
• Publication: Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 10.1016/j.cell.2014.06.045.

Backbone

• Vector backbone: pSC101
• Backbone size w/o insert (bp): 9262
• Modifications to backbone: cloned PybaJ-timerbac in pUA66 with a pSC101 replicon
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TIMER-bac
• Species: Synthetic; Discosoma spp
• Mutation: mutations: S4T and S179T
• Promoter: constitutive ybaJp promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

TIMER-bac was generated by exchanging TCC coding for serine 197 to ACC coding for threonine in DsRed.T3_S4T (Sörensen et al., 2003). PybaJ-Timer-bac was cloned into pUA66 with a pSC101 replicon, conferring kanamycin resistance.