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Addgene

TAL-L1-2.4-VP64
(Plasmid #103039)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 103039 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRN3p
  • Vector type
    T3 in vitro transcription for expression in mammalian cells

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TAL-L1-2.4-VP64
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • VP64 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BsrGI (not destroyed)
  • 5′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TAL-L1-2.4-VP64 was a gift from Maria-Elena Torres-Padilla (Addgene plasmid # 103039 ; http://n2t.net/addgene:103039 ; RRID:Addgene_103039)

  • For your References section:

    LINE-1 activation after fertilization regulates global chromatin accessibility in the early mouse embryo. Jachowicz JW, Bing X, Pontabry J, Boskovic A, Rando OJ, Torres-Padilla ME. Nat Genet. 2017 Aug 28. doi: 10.1038/ng.3945. 10.1038/ng.3945 PubMed 28846101
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