Lifeact-7-iRFP670
(Plasmid #103032)

• Purpose: Expression of iRFP670-tagged Lifeact with G418 selection in mammalian cells
• Depositing Lab: Ghassan Mouneimne
• Publication: Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi: 10.1038/s41467-018-05367-2.

Backbone

• Vector backbone: mEGFP-Lifeact
• Backbone size w/o insert (bp): 4669
• Total vector size (bp): 4720
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lifeact
• Species: Synthetic
• Insert Size (bp): 51
• Promoter: CMV
• Tag / Fusion Protein:
  • iRFP670 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Vladislav Verkhusha, Addgene #45457 Michael Davidson, Addgene #54610
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry