pLenti CMV-MRLC1-mRuby2-IRES-PuroR
(Plasmid #103031)

• Purpose: Lentiviral expression of mRuby2-tagged MRLC with puromycin selection
• Depositing Lab: Ghassan Mouneimne
• Publication: Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi: 10.1038/s41467-018-05367-2.

Backbone

• Vector backbone: pCIG3 #78264
• Backbone manufacturer: Goodrum Lab, University of Arizona
• Backbone size w/o insert (bp): 8161
• Total vector size (bp): 8677
• Modifications to backbone: Subcloned in Puromycin resistance gene in place of GFP behind the IRES sequence
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MRLC1
• Alt name: MYL9
• Species: H. sapiens (human)
• Insert Size (bp): 516
• GenBank ID: NM_006097.4
• Entrez Gene: MYL9 (a.k.a. LC20, MLC-2C, MLC2, MMIHS4, MRLC1, MYRL2)
• Promoter: CMV immediate early
• Tag / Fusion Protein:
  • mRuby2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: IRES-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Tom Egelhoff, Addgene #35680

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry