pUDE710
(Plasmid #103020)

• Purpose: expression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)
• Depositing Lab: Jean-Marc Daran
• Publication: Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):12585-12598. doi: 10.1093/nar/gkx1007.

Backbone

• Vector backbone: pUD628
• Backbone manufacturer: Świat M... Daran-Lapujade PAS FnCpf1, a novel and efficient genome editing tool for Saccharomyces cerevisiae. NAR
• Backbone size w/o insert (bp): 4995
• Total vector size (bp): 5102
• Vector type: Yeast Expression, CRISPR
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: crADE2-3.crHIS4-4.S
• gRNA/shRNA sequence: AATTTCTACTGTTGTAGATCCGGTTGTGGTATATTTGGTGTGGAAATTTCTACTGTTGTA GATGCATCTTGGCTAGCAATGAACAGAGAATTTCTACTGTTGTAGATG
• Species: S. cerevisiae (budding yeast)
• GenBank ID: NM_001183547 NM_001178675
• Entrez Gene: ADE2 (a.k.a. YOR128C)
• Entrez Gene: HIS4 (a.k.a. YCL030C)
• Promoter: SNR52

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CCGGTTGTGGTATATTTGGTG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry