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pUDC175
(Plasmid #103019)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 103019 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p414TEF1
  • Backbone manufacturer
    Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 15
  • Backbone size w/o insert (bp) 5416
  • Total vector size (bp) 9466
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fncpf1
  • Species
    Francisella tularensis subsp. novicida U112
  • Insert Size (bp)
    4038
  • Mutation
    human codon optimized
  • GenBank ID
    CP000439.1
  • Promoter TEF1
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • 3HA (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTATGAGCATCTACCAGGAGTT
  • 3′ sequencing primer TCCTTTTCGGTTAGAGCGGATGTGGGGGGAGGGCGTGAATGTAAGCGTGACATAACTAATTTAGGCATAGTCGGGGACAT
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Zetsche et al., 2015, Cell 163, 759–771. http://dx.doi.org/10.1016/j.cell.2015.09.038
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUDC175 was a gift from Jean-Marc Daran (Addgene plasmid # 103019 ; http://n2t.net/addgene:103019 ; RRID:Addgene_103019)

  • For your References section:

    FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae. Swiat MA, Dashko S, den Ridder M, Wijsman M, van der Oost J, Daran JM, Daran-Lapujade P. Nucleic Acids Res. 2017 Dec 1;45(21):12585-12598. doi: 10.1093/nar/gkx1007. 10.1093/nar/gkx1007 PubMed 29106617
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