pUDE731
(Plasmid #103008)

• Purpose: S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p
• Depositing Lab: Jean-Marc Daran
• Publication: Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):12585-12598. doi: 10.1093/nar/gkx1007.

Backbone

• Vector backbone: pMEL10
• Backbone manufacturer: Mans, R. et al (2015) CRISPR/Cas9: a molecular Swiss army knife FEMS Yeast Res 15
• Backbone size w/o insert (bp): 5658
• Total vector size (bp): 9695
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fncpf1
• Species: Francisella tularensis subsp. novicida U112
• Insert Size (bp): 4038
• Mutation: human codon optimized
• GenBank ID: CP000439.1 CP000439.1
• Promoter: TEF1
• Tags / Fusion Proteins:
  • NLS (C terminal on insert)
  • 3HA (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GCCTACGGTTCCCGAAGTATGCTGCTGATGTCTGGCTATACCTATCCGTCTACGTGAATACCCTCACTAAAGGGAACAAAAG
• 3′ sequencing primer: GTTGAACATTCTTAGGCTGGTCGAATCATTTAGACACGGGCATCGTCCTCTCGAAAGGTGGGCCGCAAATTAAAGCCTTCGAG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene plasmid # 69976--Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. 10.1016/j.cell.2015.09.038 PubMed 26422227
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry