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pZL1-ZF - myelin basic protein a (mbpa)
(Plasmid #103007)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 103007 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZL1
  • Backbone size w/o insert (bp) 4308

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The lab grows in 50ug/ml carbenicllin using DH10beta cells.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    myelin basic protein
  • Alt name
    mbp probe
  • Species
    D. rerio (zebrafish)
  • GenBank ID
    AW077205.1
  • Entrez Gene
    mbpa (a.k.a. cb274, fj33b11, mbp, wu:fj33b11, wu:fq15b02, zgc:136630)
  • Promoter Sp6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (unknown if destroyed)
  • 3′ cloning site Sal1 (unknown if destroyed)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    RZPD Berlin

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZL1-ZF - myelin basic protein a (mbpa) was a gift from Marnie Halpern (Addgene plasmid # 103007 ; http://n2t.net/addgene:103007 ; RRID:Addgene_103007)

  • For your References section:

    Characterization of myelination in the developing zebrafish. Brosamle C, Halpern ME. Glia. 2002 Jul;39(1):47-57. 10.1002/glia.10088 PubMed 12112375
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