pET22B_PcrA_2Bchimera
(Plasmid
#102998)
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PurposeExpress a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102998 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET22B
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsUSe BB834 (DE3) pLysS for protein production
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePcrA_2Bchimera
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SpeciesStaphylococcus aureus, Bacilllus stearothermophilus
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MutationBacilllus stearothermophilus PcrA in which the 2B domain has been substituted with the equivalent sequence of Staphylococcus aureus PcrA.
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySynthetically produced by GenScript (The Biology CRO, U.S.A.)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET22B_PcrA_2Bchimera was a gift from Martin Webb (Addgene plasmid # 102998 ; http://n2t.net/addgene:102998 ; RRID:Addgene_102998)
