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pLKO.1P shFXN_1
(Plasmid #102970)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102970 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1P
  • Backbone manufacturer
    Broad Institute
  • Backbone size w/o insert (bp) 7466
  • Total vector size (bp) 7518
  • Vector type
    Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shFXN_1
  • gRNA/shRNA sequence
    GCTGGACTCTTTAGCAGAGTT
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_000144
  • Entrez Gene
    FXN (a.k.a. CyaY, FA, FARR, FRDA, X25)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TRCN0000006138

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1P shFXN_1 was a gift from Richard Possemato (Addgene plasmid # 102970 ; http://n2t.net/addgene:102970 ; RRID:Addgene_102970)

  • For your References section:

    NFS1 undergoes positive selection in lung tumours and protects cells from ferroptosis. Alvarez SW, Sviderskiy VO, Terzi EM, Papagiannakopoulos T, Moreira AL, Adams S, Sabatini DM, Birsoy K, Possemato R. Nature. 2017 Nov 22. doi: 10.1038/nature24637. 10.1038/nature24637 PubMed 29168506
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