pMin1
(Plasmid
#102959)
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Purpose(Empty Backbone) Mini-transposon system suitable for sequencing, shuttle mutagenesis and gene fusions
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102959 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRH144
- Backbone size (bp) 2026
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Modifications to backboneinsertion of oriT and a MCS flanked by transcriptional terminators of bacteriophage fd (tfd) or the trpA gene (ttrpA) resulted in plasmid pMin1
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Vector typemini-transposon system suitable for sequencing, shuttling and gene fusions
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Artificial DNA for plasmid pMin1
GenBank: Z50148.1
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMin1 was a gift from Rainer Haas (Addgene plasmid # 102959 ; http://n2t.net/addgene:102959 ; RRID:Addgene_102959) -
For your References section:
An improved TnMax mini-transposon system suitable for sequencing, shuttle mutagenesis and gene fusions. Kahrs AF, Odenbreit S, Schmitt W, Heuermann D, Meyer TF, Haas R. Gene. 1995 Dec 29;167(1-2):53-7. 0378-1119(95)00671-0 [pii] PubMed 8566811
Map uploaded by the depositor.
