pHMR1B
(Plasmid
#102958)
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Purposegene cloning of MR1B
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102958 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR2.1-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3908
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Vector typeCloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMR1B cDNA
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Alt nameAF010446
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SpeciesH. sapiens (human)
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Insert Size (bp)752
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MutationStop codon has been removed by HindIII site
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Entrez GeneMR1P1 (a.k.a. MR1B)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Reference: Riegert et al. J. Immunol. 1998, 161:4066-4077
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHMR1B was a gift from Gennaro De Libero & Lucia Mori (Addgene plasmid # 102958 ; http://n2t.net/addgene:102958 ; RRID:Addgene_102958)