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Addgene

pJH124
(Plasmid #102951)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102951 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS425
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 7500
  • Total vector size (bp) 8500
  • Modifications to backbone
    GAL1-inducible promoter / GAL4 terminator
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    APEX2-NES
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    786
  • GenBank ID
    Addgene Plasmid #49386
  • Promoter GAL1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH124 was a gift from Peter Espenshade (Addgene plasmid # 102951 ; http://n2t.net/addgene:102951 ; RRID:Addgene_102951)

  • For your References section:

    Proximity-dependent biotin labeling in yeast using the engineered ascorbate peroxidase APEX2. Hwang J, Espenshade PJ. Biochem J. 2016 Jun 7. pii: BCJ20160106. 10.1042/BCJ20160106 PubMed 27274088
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