pCMV-5U-Venus-PSD-95-3U
(Plasmid #102949)

• Purpose: Expresses Venus-PSD-95 in mammalian cells
• Depositing Lab: Gary Bassell
• Publication: Ifrim et al J Neurosci. 2015 May 6;35(18):7116-30. doi: 10.1523/JNEUROSCI.2802-14.2015.

Backbone

• Vector backbone: pCMV
• Backbone size w/o insert (bp): 4141
• Total vector size (bp): 8304
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PSD-95
• Alt name: DLG4, SAP-90
• Species: M. musculus (mouse)
• GenBank ID: NC_000077.5
• Entrez Gene: Dlg4 (a.k.a. Dlgh4, PSD-95, PSD95, SAP90, SAP90A)
• Promoter: CMV
• Tag / Fusion Protein:
  • Venus Fluorescent protein (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: kpnI (not destroyed)
• 5′ sequencing primer: CGCAAATGGGCGGTAGGCGTG
• 3′ sequencing primer: GGAACAACACTCAACCCTATCT

Resource Information

• Supplemental Documents:
  • pCMV-5U-Venus-PSD-95-3U-2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry