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Addgene

pRTS-GBP-dCas9-mRFP
(Plasmid #102934)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102934 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    prTS
  • Backbone manufacturer
    Bornkamm , et al. PMID 16147984
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9
  • Species
    Synthetic
  • Tags / Fusion Proteins
    • GBP (GFP binding protein (N terminal on insert)
    • mRFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (unknown if destroyed)
  • 3′ cloning site SfiI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRTS-GBP-dCas9-mRFP was a gift from Sebastian Bultmann (Addgene plasmid # 102934 ; http://n2t.net/addgene:102934 ; RRID:Addgene_102934)

  • For your References section:

    Site-specific recruitment of epigenetic factors with a modular CRISPR/Cas system. Anton T, Bultmann S. Nucleus. 2017 May 4;8(3):279-286. doi: 10.1080/19491034.2017.1292194. Epub 2017 Feb 23. 10.1080/19491034.2017.1292194 PubMed 28448738
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