pRTS-GBP-dCas9-mRFP
(Plasmid
#102934)
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PurposeTetracyclin inducible expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102934 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneprTS
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Backbone manufacturerBornkamm , et al. PMID 16147984
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9
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SpeciesSynthetic
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Tags
/ Fusion Proteins
- GBP (GFP binding protein (N terminal on insert)
- mRFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SfiI (unknown if destroyed)
- 3′ cloning site SfiI (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRTS-GBP-dCas9-mRFP was a gift from Sebastian Bultmann (Addgene plasmid # 102934 ; http://n2t.net/addgene:102934 ; RRID:Addgene_102934) -
For your References section:
Site-specific recruitment of epigenetic factors with a modular CRISPR/Cas system. Anton T, Bultmann S. Nucleus. 2017 May 4;8(3):279-286. doi: 10.1080/19491034.2017.1292194. Epub 2017 Feb 23. 10.1080/19491034.2017.1292194 PubMed 28448738