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Addgene

pCAG-GBP-dCas9-mRFP
(Plasmid #102933)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102933 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9
  • Species
    Synthetic
  • Tags / Fusion Proteins
    • GBP (GFP binding protein) (N terminal on insert)
    • mRFP (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The GBP (GFP binding protein) and mRFP coding sequences were amplified from pGFPbinderImR (PMID 18622019) and cloned into pCAG-dCas9 (PMID 24637835) via XbaI and AsiSI/NotI, respectively.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-GBP-dCas9-mRFP was a gift from Sebastian Bultmann (Addgene plasmid # 102933 ; http://n2t.net/addgene:102933 ; RRID:Addgene_102933)

  • For your References section:

    Site-specific recruitment of epigenetic factors with a modular CRISPR/Cas system. Anton T, Bultmann S. Nucleus. 2017 May 4;8(3):279-286. doi: 10.1080/19491034.2017.1292194. Epub 2017 Feb 23. 10.1080/19491034.2017.1292194 PubMed 28448738
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