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Addgene

pLJC5-LAMP1-RFP-2xFlag
(Plasmid #102931)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102931 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLJC5
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LAMP1
  • Species
    R. norvegicus (rat)
  • Promoter ubc
  • Tag / Fusion Protein
    • mRFP-2xFlag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcorI (not destroyed)
  • 5′ sequencing primer CGAAGGAATAGAAGAAGAAGGTGGAGA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

LAMP1-RFP was cloned from Plasmid #34611

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLJC5-LAMP1-RFP-2xFlag was a gift from David Sabatini (Addgene plasmid # 102931 ; http://n2t.net/addgene:102931 ; RRID:Addgene_102931)

  • For your References section:

    Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent regulation of amino acid efflux from lysosomes. Abu-Remaileh M, Wyant GA, Kim C, Laqtom NN, Abbasi M, Chan SH, Freinkman E, Sabatini DM. Science. 2017 Nov 10;358(6364):807-813. doi: 10.1126/science.aan6298. Epub 2017 Oct 26. 10.1126/science.aan6298 PubMed 29074583
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