pCKTRBS-OD
(Plasmid
#102924)
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Purpose(Empty Backbone) pCK01 derivative carrying tetR under a constitutive promoter and an aTc-inducible PtetO promoter for control expression of genes. Contains LacI OD for translational fusions on the Ct of cloned genes.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102924 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCK01
- Backbone size (bp) 3679
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Modifications to backboneRemove Plac promoter. Introduce PtetO promoter and a consensus RBS for expression in Escherichia coli. Introduce tetR (and a promoter to express it). Introduce LacI OligomerizatioN Domain region (-OD).
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Vector typeBacterial Expression
- Promoter PtetO
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCKTRBS-OD was a gift from George Church (Addgene plasmid # 102924 ; http://n2t.net/addgene:102924 ; RRID:Addgene_102924) -
For your References section:
Biosensor libraries harness large classes of binding domains for construction of allosteric transcriptional regulators. Juarez JF, Lecube-Azpeitia B, Brown SL, Johnston CD, Church GM. Nat Commun. 2018 Aug 6;9(1):3101. doi: 10.1038/s41467-018-05525-6. 10.1038/s41467-018-05525-6 PubMed 30082754
Map uploaded by the depositor.
