pCMV-ABE6.3
(Plasmid #102916)

• Purpose: expresses ABE6.3 in mammalian cells
• Depositing Lab: David Liu
• Publication: Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25.

Backbone

• Vector backbone: pBR322/CMV
• Backbone manufacturer: Liu lab
• Backbone size w/o insert (bp): 3388
• Total vector size (bp): 8727
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ABE6.3
• Alt name: TadA-linker(32aa)-TadA*(6.3)-linker(32aa)-nCas9-NLS
• Species: Synthetic; TadA is from E.coli (synthetic gene block, mammalian codon optimized), Cas9 is S. pyogenes (mammalian codon optimized)
• Insert Size (bp): 5328
• Mutation: see manuscript
• Promoter: CMV
• Tag / Fusion Protein:
  • NLS (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: M13

Resource Information

• Supplemental Documents:
  • ABE6.3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry