PB-EEA-5g-OSK2M2L1-PGK-Puro
(Plasmid #102909)

• Purpose: PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.
• Depositing Lab: Timo Otonkoski
• Publication: Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.

Backbone

• Vector backbone: PB-GG-PGK-puro
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EEA-5g-OSK2M2L1-gRNAs-PGK-Puro
• gRNA/shRNA sequence: 15 gRNAs, see full sequence
• Species: H. sapiens (human)
• Entrez Gene: POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
• Entrez Gene: SOX2 (a.k.a. ANOP3, MCOPS3)
• Entrez Gene: KLF4 (a.k.a. EZF, GKLF)
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
• Entrez Gene: LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • PB-GG-EEA-5g-OSK2M2L1-PGK-Puro
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry