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pGEX-PGC1(1-400)
(Plasmid #1029)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1029 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-5x-2
  • Backbone manufacturer
    Amersham Biosciences
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGC-1 alpha
  • Alt name
    PGC1
  • Alt name
    PGC-1
  • Alt name
    PPAR gamma coactivator 1 alpha
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • Mutation
    contains only aa1-400
  • GenBank ID
    NM_008904
  • Entrez Gene
    Ppargc1a (a.k.a. A830037N07Rik, Gm11133, PGC-1, PPARGC-1-alpha, Pgc-1alpha, Pgc1, Pgco1, Ppargc1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pGEX5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-PGC1(1-400) was a gift from Bruce Spiegelman (Addgene plasmid # 1029 ; http://n2t.net/addgene:1029 ; RRID:Addgene_1029)

  • For your References section:

    Activation of PPARgamma coactivator-1 through transcription factor docking. Puigserver P, Adelmant G, Wu Z, Fan M, Xu J, O'Malley B, Spiegelman BM. Science 1999 Nov 12;286(5443):1368-71. 10.1126/science.286.5443.1368 PubMed 10558993
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