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Addgene

pLEX307-mFzd9
(Plasmid #102877)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102877 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEX_307
  • Backbone manufacturer
    Addgene #41392 (provided by David Root)
  • Backbone size w/o insert (bp) 10065
  • Total vector size (bp) 10505
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Frizzled 9
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1779
  • Mutation
    A to G substitution at nucleotide 114, G to A substitution at nucleotide 999 of Fzd9 ORF; both substitutions are silent and do not alter amino acid sequence
  • Entrez Gene
    Fzd9 (a.k.a. mfz9)
  • Promoter EF1A

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert was PCR amplified from Addgene plasmid #42261 (provided by Jeremy Nathans and Chris Garcia) and subcloned into a modified pENTR-2B vector using FseI and AscI sites.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX307-mFzd9 was a gift from Henry Ho (Addgene plasmid # 102877 ; http://n2t.net/addgene:102877 ; RRID:Addgene_102877)

  • For your References section:

    Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrates. Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R, Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH. Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509. 10.7554/eLife.26509 PubMed 28885975
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