strep-IRF8
(Plasmid
#102872)
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PurposeExpress strep-tagged mouse IRF8 protein in E. coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102872 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneNEB DHFR control plasmid
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIRF8
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SpeciesM. musculus (mouse)
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Entrez GeneIrf8 (a.k.a. ICSBP, IRF-8, Icsbp1, Myls)
- Promoter T7
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Tag
/ Fusion Protein
- strep tag (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
strep-IRF8 was a gift from Gary Stormo (Addgene plasmid # 102872 ; http://n2t.net/addgene:102872 ; RRID:Addgene_102872) -
For your References section:
Quantitative profiling of BATF family proteins/JUNB/IRF hetero-trimers using Spec-seq. Chang YK, Zuo Z, Stormo GD. BMC Mol Biol. 2018 Mar 27;19(1):5. doi: 10.1186/s12867-018-0106-7. 10.1186/s12867-018-0106-7 PubMed 29587652
Map uploaded by the depositor.
