pLEX307 hDVL1
(Plasmid
#102863)
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PurposeExpresses human DVL1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102863 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLEX_307
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Backbone manufacturerAddgene #41392 (provided by David Root)
- Backbone size w/o insert (bp) 10065
- Total vector size (bp) 10731
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman Dishevelled 1 (DVL1)
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SpeciesH. sapiens (human)
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MutationChanged alanine 2 to glycine. Likely due to polymorphism, as sequence AAB65242.1 has the same substitution
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GenBank IDNM_004421.2
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Entrez GeneDVL1 (a.k.a. DRS2, DVL, DVL1L1, DVL1P1)
- Promoter EF1A
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EF1A promoter forward (TCAAGCCTCAGACAGTGGTTC) (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEX307 hDVL1 was a gift from Henry Ho (Addgene plasmid # 102863 ; http://n2t.net/addgene:102863 ; RRID:Addgene_102863) -
For your References section:
Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrates. Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R, Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH. Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509. 10.7554/eLife.26509 PubMed 28885975