hisSUMO-CTCF(F1-F9)
(Plasmid #102859)

• Purpose: Express DNA binding domain of mouse CTCF in E. coli
• Depositing Lab: Gary Stormo
• Publication: Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi: 10.1126/sciadv.aao1799. eCollection 2017 Nov.

Backbone

• Vector backbone: NEB DHFR control plasmid
• Total vector size (bp): 3447
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CTCF
• Species: M. musculus (mouse)
• Insert Size (bp): 849
• Entrez Gene: Ctcf
• Promoter: T7
• Tag / Fusion Protein:
  • hisSUMO (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • hisSUMO-CTCF(mouse)-F1-F9.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid encodes the DNA binding domain of mouse CTCF protein (F1 to F9) for E. coli expression.