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Addgene

tdTomato-KIF1A
(Plasmid #102850)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102850 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    peGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 9902
  • Modifications to backbone
    EGFP replaced by tdTomato
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KIF1A
  • Alt name
    ATSV, Kns1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    5085
  • Entrez Gene
    Kif1a (a.k.a. A, ATSV, C630002N23Rik, Gm1626, Kn, Kns1)
  • Promoter CMV
  • Tag / Fusion Protein
    • tdTomato (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI or AgeI (unknown if destroyed)
  • 3′ cloning site ScaI (unknown if destroyed)
  • 5′ sequencing primer tdTomato-Fwd
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The rat eGFP-KIF1A was a kind gift from Gary Banker (OHSU).

The tdTomato-KIF1A plasmid was constructed by restriction site removal of the eGFP from the eGFP-KIF1A plasmid and insertion of tdTomato.

Addgene NGS results found S33N within the KIF1A translation compared to NCBI reference sequence XP_017459420.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tdTomato-KIF1A was a gift from Erik Dent (Addgene plasmid # 102850 ; http://n2t.net/addgene:102850 ; RRID:Addgene_102850)

  • For your References section:

    Transport of a kinesin-cargo pair along microtubules into dendritic spines undergoing synaptic plasticity. McVicker DP, Awe AM, Richters KE, Wilson RL, Cowdrey DA, Hu X, Chapman ER, Dent EW. Nat Commun. 2016 Sep 23;7:12741. doi: 10.1038/ncomms12741. 10.1038/ncomms12741 PubMed 27658622
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