pET28a-SpyTag002-MBP
(Plasmid #102831)

• Purpose: Bacterial expression of SpyTag002 fused to maltose binding protein
• Depositing Lab: Mark Howarth
• Publication: Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi: 10.1002/anie.201707623.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpyTag002-MBP
• Species: Synthetic
• Insert Size (bp): 1176
• Mutation: Peptide tag added to the N-terminus of MBP via a GSGESG linker
• Promoter: T7
• Tags / Fusion Proteins:
  • His6 (N terminal on backbone)
  • Thrombin cleavage site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7F
• 3′ sequencing primer: T7R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry