pDEST14-SpyCatcher002 EQ
(Plasmid #102830)

• Purpose: Bacterial expression of unreactive version of SpyCatcher002
• Depositing Lab: Mark Howarth
• Publication: Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi: 10.1002/anie.201707623.

Backbone

• Vector backbone: pDEST14
• Backbone manufacturer: Thermo Fisher Scientific
• Backbone size w/o insert (bp): 6422
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpyCatcher002 EQ
• Species: Synthetic
• Insert Size (bp): 435
• Mutation: E77Q mutation prevents isopeptide bond formation
• Promoter: T7
• Tags / Fusion Proteins:
  • His6 (N terminal on backbone)
  • TEV cleavage site (N terminal on insert)
  • GSSGS (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7F
• 3′ sequencing primer: T7R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry