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PurposeBacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102828 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDEST14
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Backbone manufacturerThermo Fisher Scientific
- Backbone size w/o insert (bp) 6422
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAvitag-SpyCatcher002
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SpeciesSynthetic
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Insert Size (bp)489
- Promoter T7
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Tags
/ Fusion Proteins
- His6 (N terminal on backbone)
- Avitag (N terminal on insert)
- TEV cleavage site (N terminal on insert)
- GSSGS (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST14-Avitag-SpyCatcher002 was a gift from Mark Howarth (Addgene plasmid # 102828 ; http://n2t.net/addgene:102828 ; RRID:Addgene_102828) -
For your References section:
Evolving accelerated amidation by SpyTag/SpyCatcher to analyze membrane dynamics. Keeble AH, Banerjee A, Reddington SC, Ferla MP, Howarth M, Khairil Anuar INA. Angew Chem Int Ed Engl. 2017 Oct 10. doi: 10.1002/anie.201707623. 10.1002/anie.201707623 PubMed 29024296
