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Addgene

Lv EF1a dCas9-HA 2A Blast
(Plasmid #102812)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102812 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-HA
  • Mutation
    D10A and N863A (dCas9)
  • Promoter EF1a

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    modified from addgene plasmid #61425

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lv EF1a dCas9-HA 2A Blast was a gift from Jerry Crabtree (Addgene plasmid # 102812 ; http://n2t.net/addgene:102812 ; RRID:Addgene_102812)

  • For your References section:

    Rapid and reversible epigenome editing by endogenous chromatin regulators. Braun SMG, Kirkland JG, Chory EJ, Husmann D, Calarco JP, Crabtree GR. Nat Commun. 2017 Sep 15;8(1):560. doi: 10.1038/s41467-017-00644-y. 10.1038/s41467-017-00644-y [pii] PubMed 28916764
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