Lv EF1a HP1cs-Frb1x PGK Puro
(Plasmid
#102808)
-
PurposeLentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb1x fusion protein
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102808 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepLenti
-
Vector typeMammalian Expression, Lentiviral, CRISPR
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameHP1cs-Frb1x
- Promoter EF1a
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer EF1a Fw
- 3′ sequencing primer PGK Rv (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made bymodified from addgene plasmid #44195
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Lv EF1a HP1cs-Frb1x PGK Puro was a gift from Jerry Crabtree (Addgene plasmid # 102808 ; http://n2t.net/addgene:102808 ; RRID:Addgene_102808) -
For your References section:
Rapid and reversible epigenome editing by endogenous chromatin regulators. Braun SMG, Kirkland JG, Chory EJ, Husmann D, Calarco JP, Crabtree GR. Nat Commun. 2017 Sep 15;8(1):560. doi: 10.1038/s41467-017-00644-y. 10.1038/s41467-017-00644-y [pii] PubMed 28916764
Map uploaded by the depositor.
