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PurposeLentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp2x fusion protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102807 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMS2-Fkbp2x
- Promoter EF1a
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer EF1a Fw
- 3′ sequencing primer WPRE Rv (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bymodified from addgene plasmid #61426
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lv EF1a MS2-Fkbp2x 2A Hygro was a gift from Jerry Crabtree (Addgene plasmid # 102807 ; http://n2t.net/addgene:102807 ; RRID:Addgene_102807) -
For your References section:
Rapid and reversible epigenome editing by endogenous chromatin regulators. Braun SMG, Kirkland JG, Chory EJ, Husmann D, Calarco JP, Crabtree GR. Nat Commun. 2017 Sep 15;8(1):560. doi: 10.1038/s41467-017-00644-y. 10.1038/s41467-017-00644-y [pii] PubMed 28916764