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pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP
(Plasmid #102798)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102798 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV
  • Vector type
    Mammalian Expression, Retroviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    sgRNA
  • Alt name
    GAAGTTCGAGGGCGACACCC
  • Species
    Synthetic

Gene/Insert 2

  • Gene/Insert name
    EGFP
  • Mutation
    H231L

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by gibson assembly from pKLV2(gfp)U6sgRNA5(BbsI)-PGKpuroBFP and pMSCV-IRES-mCherry FP. Used to verify CRISPR activity

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP was a gift from Sarah Teichmann (Addgene plasmid # 102798 ; http://n2t.net/addgene:102798 ; RRID:Addgene_102798)

  • For your References section:

    Genome-wide CRISPR Screens in T Helper Cells Reveal Pervasive Crosstalk between Activation and Differentiation. Henriksson J, Chen X, Gomes T, Ullah U, Meyer KB, Miragaia R, Duddy G, Pramanik J, Yusa K, Lahesmaa R, Teichmann SA. Cell. 2019 Jan 7. pii: S0092-8674(18)31569-1. doi: 10.1016/j.cell.2018.11.044. 10.1016/j.cell.2018.11.044 PubMed 30639098
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