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Addgene

pCLXSN-EphA2-Flag
(Plasmid #102755)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102755 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCLXSN
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EphA2
  • Species
    H. sapiens (human)
  • Entrez Gene
    EPHA2 (a.k.a. ARCC2, CTPA, CTPP1, CTRCT6, ECK)
  • Tag / Fusion Protein
    • Flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer pBABE 5 primer
  • 3′ sequencing primer pBABE 3 primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCLXSN-EphA2-Flag was a gift from Jin Chen (Addgene plasmid # 102755 ; http://n2t.net/addgene:102755 ; RRID:Addgene_102755)

  • For your References section:

    JNK signaling mediates EPHA2-dependent tumor cell proliferation, motility, and cancer stem cell-like properties in non-small cell lung cancer. Song W, Ma Y, Wang J, Brantley-Sieders D, Chen J. Cancer Res. 2014 May 1;74(9):2444-54. doi: 10.1158/0008-5472.CAN-13-2136. Epub 2014 Mar 7. 10.1158/0008-5472.CAN-13-2136 PubMed 24607842
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