G766E EPHA3 pcDNA3.1
(Plasmid #102753)

• Purpose: Expression of EPHA3 in mammalian cells
• Depositing Lab: Jin Chen
• Publication: Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1182-97. doi: 10.1093/jnci/djs297. Epub 2012 Jul 24.

Backbone

• Vector backbone: pcDNA3.1(+)/myc-His B
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EPHA3
• Alt name: MEK4
• Alt name: TYRO4
• Species: H. sapiens (human)
• Mutation: G766E (GGA > GaA)
• Entrez Gene: EPHA3 (a.k.a. EK4, ETK, ETK1, HEK, HEK4, TYRO4)
• Promoter: CMV
• Tag / Fusion Protein:
  • myc-6xhis (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note last 10 amino acids in C-terminal are omitted in these constructs. These series of EPHA3 constructs carry missense mutations found in lung and colon cancer.